>From usenet.ucs.indiana.edu!ux1.cso.uiuc.edu!sdd.hp.com!zaphod.mps.ohio-state.edu!darwin.sura.net!haven.umd.edu!uunet!stanford.edu!rutgers!mcclb0.med.nyu.edu!kleinschmidt Thu Sep 17 06:50:00 EST 1992 Article: 663 of bionet.software Path: usenet.ucs.indiana.edu!ux1.cso.uiuc.edu!sdd.hp.com!zaphod.mps.ohio-state.edu!darwin.sura.net!haven.umd.edu!uunet!stanford.edu!rutgers!mcclb0.med.nyu.edu!kleinschmidt >From: kleinschmidt@mcclb0.med.nyu.edu Newsgroups: bionet.software Subject: Calcium buffer program available for ftp Message-ID: <1992Sep16.222646.7146@mcclb0.med.nyu.edu> Date: 17 Sep 92 03:26:45 GMT Organization: NYU Medical Center, New York, NY 10016, USA Lines: 91 CABUFFER program The program CABUFFER is available by anonymous ftp from mcclb0.med.nyu.edu [128.122.135.4]. This program allows you to calculate the concentrations of all ionic species present in a mixture of up to four divalent cations (e.g. Mg, Ca, Sr, Ba) and four ligands ("buffers") for these ions. Examples of such buffers are EDTA, EGTA, NTA, HEDTA, citrate, Ca-binding proteins, etc. Corrections for temperature, ionic stength and pH are provided. The program is written in structured Basic and has been compiled with Borland's Turbo Basic v. 1.0. It can also be compiled with Microsoft's Quickbasic v. 4.0 or run from within the QBASIC program provided with MS-DOS 5.0 or higher, after making a few changes (see the readme file). The compiled program runs on PC's under PC-DOS/MS-DOS 2.0 or higher. It runs very fast on PC's outfitted with a math coprocessor. A somewhat less capable program, CABUF, that runs reasonably fast even on machines without math coprocessor, is also available. Both executable files and Basic source code is included. All the files take up about 280 Kb of space, including a readme file that tells you how to use the program. The CABUFFER.EXE file is about 80 Kb long. Originally, I meant to write a paper on the use of Ca-buffers in physiology and on the traps one can fall into using them. The manuscript was never completed but drafts of various sections are included with CABUFFER. They may make instructive reading for those who care to understand what they are doing when they make up and use Ca-buffers. RESULTS.TXT, in particular, describes the basic aspects of the CABUFFER program, including the corrections utilized. I apologize for the fact that equations are missing from the electronic version of the manuscript; I couldn't get them into ASCII format. Unfortunately, I have little time to support this program. If you use it, you do so at your own risk. But I believe that its use is self-explanatory and easy, and I also think that it is largely bugfree. The program is supplied in two files: CABUFZIP.EXE: This is a binary, self-extracting, compressed archive file that contains all program, data and text files. CABUFZIP.RME: A short readme file that gives installation instructions for CABUFFER. This file is reproduced below. FTP instructions At the command prompt of your machine, type: ftp 128.122.135.4 This will connect you to my host machine (mcclb0.med.nyu.edu). Follow the prompts as shown below. Name: anonymous Password: your_user_name ftp> binary ftp> get cabufzip.exe ftp> ascii ftp> get cabufzip.rme ftp> quit -------------------------------------- CABUFZIP.RME (9-12-92) Installation instructions for CABUFFER on MS-DOS machines The compiled CABUFFER program, Basic source code files, data files, and explanatory text files all reside in one self-extracting, compressed archive file named cabufzip.exe. To install all of these files on your PC-DOS/MS-DOS machine, at the ftp prompt type BINARY to set the transfer mode to binary, then transfer the file with the command GET CABUFZIP.EXE . Once this file is on your PC, all you have to do is to execute it with the command CABUFZIP , preferably in its own subdirectory. This will extract and unpack the compressed component files. The cabufzip.exe file is 125 kB long, the extracted files add up to about 280 kB. CABUFFER itself is 80 Kb long. The user notes for CABUFFER are in the readme file. Jochen Kleinschmidt, Ph.D. Dept. of Ophthalmology NYU Medical Center Phone: 212-263-5530 Internet: kleinschmidt@mcclb0.med.nyu.edu Bitnet: kleinschmidt@nyumed.bitnet